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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is implementing a new quality control procedure for blood culture media.
To ensure the media supports the growth of a wide range of clinically significant organisms, they should include:

A) A diverse panel of fastidious and non-fastidious bacteria and yeasts.
B) Only Gram-positive and Gram-negative control strains.
C) Only antibiotic-resistant control strains.
D) Only control strains that are commonly found as contaminants.

2. A patient with a history of intravenous drug use develops a bloodstream infection with Staphylococcus aureus. Antimicrobial susceptibility testing reveals resistance to methicillin. The mechanism of methicillin resistance in Staphylococcus aureus is primarily mediated by:

A) Increased expression of efflux pumps.
B) Alteration of penicillin-binding proteins (PBPs).
C) Acquisition of genes encoding aminoglycoside-modifying enzymes.
D) Production of beta-lactamase enzymes.

3. Which biosafety level (BSL) is required for clinical laboratory work involving the propagation (culture) of Coccidioides immitis/posadasii?

A) BSL-4
B) BSL-1
C) BSL-3
D) BSL-2

4. A Gram stain of anaerobic growth from a peritoneal fluid abscess reveals pleomorphic, pale-staining Gram- negative rods. The isolate grows on Bacteroides Bile Esculin (BBE) agar, hydrolyzing esculin (producing black colonies), and is resistant to kanamycin (1000 µg), vancomycin (5 µg), and colistin (10 µg) disks. Which organism group is most likely?

A) Prevotella melaninogenica group
B) Fusobacterium nucleatum
C) Bacteroides fragilis group
D) Clostridium perfringens

5. A patient with a history of travel to South America presents with fever, lymphadenopathy, and hepatosplenomegaly. Peripheral blood smear reveals the presence of trypomastigotes. The MOST likely diagnosis is:

A) Malaria
B) Chagas disease (American trypanosomiasis)
C) Leishmaniasis
D) African trypanosomiasis (sleeping sickness)

Solutions: